1. Prepare WBC Dilution
Teaching example uses 1:20 dilution. Follow your SOP for actual testing.
WBC Diluting Fluid
EDTA Blood
1:20 Dilution
20
Virtual pipette
2. Charge Neubauer Chamber
Large Square 1
?
Large Square 2
?
Large Square 3
?
Large Square 4
?
Use the same boundary rule consistently to avoid double counting.
3. Record Counts & Calculate TLC
Square 1
Square 2
Square 3
Square 4
For 4 large squares, chamber volume = 0.4 µL. TLC = cells counted × dilution ÷ 0.4.
Complete counting first.