1. Interactive Gram Stain Bench
Drag the slide and the reagent bottles / wash bottle / burner with your mouse. When the correct object is placed in the working zone, click the matching action button to perform the step.
Start by dragging the slide to the staining rack, then click “Prepare Smear”.
Staining Rack
Crystal Violet
Gram Iodine
Decolorizer
Safranin
Wash Bottle
Bunsen Burner
Microscope
2. Principle, Reagents & Procedure
Principle
Gram-positive organisms retain the crystal violet–iodine complex after controlled decolorization and appear purple. Gram-negative organisms lose the primary stain on decolorization and take up the counterstain, appearing pink/red.
Reagents
- Crystal violet
- Gram iodine
- Decolorizer
- Safranin
- Clean water / wash bottle
Correct order
- Prepare thin smear
- Heat fixation
- Crystal violet
- Wash
- Gram iodine
- Wash
- Decolorize
- Wash immediately
- Safranin
- Wash and dry
- Microscopy
3. Microscope View & Interpretation
After the stain is complete, drag the slide to the microscope and click the button below. The teaching case opens a selectable microscopy field.
🔬 DIGITAL MICROSCOPE
READY FOR STAINED SLIDE
4. Practice Report
Complete the practical and save your microscopy interpretation.